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PCR confirmation of the pPICZαA-ZIKV-EDIII construct and transformation of K. phaffii. (a) PCR of pPICZαA-ZIKV-EDIII confirming the correct cloning of the cassette on the vector. M: molecular weight marker; 1: amplified fragment of pPICZαA-ZIKV-EDIII; 2: empty pPICZαA used as the negative control. (b) PCR of K. phaffii genomic DNA. M: molecular weight marker; 1–3: transformed K. phaffii clones; 4: untransformed K. phaffii (negative control).
