Table 1.

In vitro profiling of ZED1227.

Molecular Mass [g/mol]	528.6
Solubility at pH 7.4 Solubility at pH 1.1	100 μM/0.05 g/L 48,900 μM/25.9 g/L
LogD (octanol/PBS pH 7.4)	2.0
Stability in PBS/saline (24 h, 37 °C)	10% at pH 7.4 89% at pH 5.0
Stability in artificial gastric and intestinal fluid (24 h, 37 °C)	95%, simulated gastric fluid (pH 1.2) 82%, simulated intestinal fluid (pH 6.5)
A-B permeability (Caco-2-assay)	Papp < 1 × 10−6 cm/s
Cytotoxicity Proliferation: Caco-2 cells       Huh7 cells   Viability: Caco-2 cells       Huh7 cells	no effect up to 1,000 μM no effect up to 1,000 μM no effect up to 1,000 μM no effect up to 1,000 μM
Reactivity towards excess glutathione (25 °C)	98% parent drug compound recovery (after 48 h)