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Overexpression of Rcd from the single-shot vector. DS941hns-205 transformed with the single-shot vector, pCm(ss)-19 (●), was grown initially in L broth at 30°C and then diluted in L broth prewarmed to 42°C at t = 0. Cell viability was tested by the ability to form colonies on Iso-Sensitest broth agar plates with appropriate antibiotic selection for pCm(ss)-19 under repressing conditions (30°C) for Rcd expression (■).
