FIG. 5.

Assessment of the de novo protein synthetic capacity of quiescent cells. Samples (10 ml) from a culture of DS941hns-205 pCm(ss)-19 were labelled for 1 h with [³⁵S]methionine at various times after induction of Rcd expression. Total proteins were separated on an SDS–4 to 15% polyacrylamide gradient gel, and [³⁵S]methionine labelling was revealed by autoradiography. Lanes: 1, Rainbow ¹⁴C-methylated protein molecular weight markers; 2, DS941hns-205 pCm(ss)-19 grown at 30°C for 1 h (OD₆₀₀ = 0.359); 3, DS941hns-205 grown at 42°C for 1 h (OD₆₀₀ = 0.225); 4, DS941hns-205 pCm(ss)-19 grown at 42°C for 1 h (OD₆₀₀ = 0.147); 5, DS941hns-205 pCm(ss)-19 grown at 42°C for 2 h (OD₆₀₀ = 0.211); 6, DS941hns-205 pCm(ss)-19 grown at 42°C for 7 h (OD₆₀₀ = 0.279); 7, DS941hns-205 pCm(ss)-19 grown at 30°C for 10 h (OD₆₀₀ = 0.285). An equal volume of total protein preparation was loaded in each lane.