FIG. 6.

Analysis of total protein isolated from quiescent cells. Total protein from DS941hns-205 pCm(ss)-19 was isolated at various times after the induction of Rcd expression. Proteins were separated on an SDS–12.5% polyacrylamide gel and stained with Coomassie brilliant blue R. Lanes: 1, purified CAT protein (0.4 μg); 2, total protein from DS941hns-205 pACYC/cIts857 grown at 42°C for 160 min (OD₆₀₀ = 0.219); 3, DS941hns-205 pCm(ss)-19 grown at 42°C for 1 h (OD₆₀₀ = 0.147); 4, for 2 h (OD₆₀₀ = 0.211); 5, for 7 h (OD₆₀₀ = 0.279); or 6, for 10 h (OD₆₀₀ = 0.285).