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. 2022 May 13;13:819826. doi: 10.3389/fphar.2022.819826

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Copyright © 2022 Li, Yang, Yuan, Lin, Tian, Li, Zhang, Wang, Xie, Wang and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IR alleviates intestinal mucosal barrier damage in UC mice. (A) Detection of acidic mucus layer in colon of mice by alcian blue staining (n = 6). (B) The LPS content in serum of mice (n = 6). (C) The FITC-dextran content in serum of mice (n = 6). (D) Immunohistochemical detection of E-cadherin protein expression in colon of mice (n = 3). (E) Histochemical positive area statistics of E-cadherin (n = 3). (F) The protein levels of ZO-1, claudin-1, and occludin in colon detected by western blotting. (G–I) The gray intensity analysis of ZO-1, claudin-1, and occludin proteins, respectively (n = 3). All data were compared using one-way ANOVA, and p-values reflected differences between experimental groups.