Figure 6.

AITC-mediated modulation of potassium currents in transfected HEK293 cells. (A,B) IV relations of I_K in HEK-Slack cells ((A); n = 5 cells) and HEK-Slack-TRPA1 cells ((B); n = 14 cells) obtained in whole-cell patch-clamp recordings using a Ca²⁺-free external solution before and after TRPA1 activation by 200 µM AITC. (C) IV relations of I_K in HEK-Slack-TRPA1 cells before and after TRPA1 inhibition by 10 µM A-967079 (n = 9 cells) in whole-cell patch-clamp recordings using a Ca²⁺-free external solution. (D) IV relations of I_K in HEK-Slack-TRPA1 cells before and after 200 µM AITC application (n = 9 cells) in whole-cell patch-clamp recordings in physiological extracellular buffer Note that in a Ca²⁺-free external solution, Slack-mediated I_K is increased after TRPA1 activation. Data are presented as mean ± SEM. Paired t test, * p < 0.05.