FIG. 2.

DNA fingerprint patterns of representative Pseudomonas isolates typed with the primer RW3A. PCR products (10-μl volumes) were loaded onto a 2% agarose gel in 1× Tris-acetate-EDTA buffer containing 0.1 μg of ethidium bromide per ml. Samples were electrophoresed at 100 V for 90 min. Lanes M contain molecular weight markers (grade III; Boehringer). Lane M* contains a mixture of molecular weight markers (grades III and V). Lane 1, ATCC 27853 (group I); lane 2, CIT-V2 (II); lane 3, CIT-V3 (II); lane 4, CIT-V4 (II); lane 5, CIT-V15 (III); lane 6, CIT-H1 (IV); lane 7, CIT-H2 (V); lane 8, CIT-H3 (VI); lane 9, CIT-H5 (VII); lane 10, CIT-H6 (VIII); lane 11, CIT-H7 (IX); lane 12, CIT-H8 (X); lane 13, CIT-H9 (VI); lane 14, CIT-H10 (XI); lane 15, CIT-H11 (XII); lane 16, CIT-H12 (XIII); and lane 17, CIT-H13 (XIV). Numbers in parentheses denote DAF groups.