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. 2022 Apr 22;13(5):732. doi: 10.3390/genes13050732

Table 2.

Primer sequences used in RT-qPCR reaction (F—Forward primer; R—Reverse primer).

Gene Name Primer Sequences Ref.
ACTB Actin β F: CACAGATCATGTTTGAGACCTT
R: CATCACAATACCAGTGGTACG
Adapted from Ref. [17]
G6PDH Glucose-6-phosphate dehydrogenase F: CGGGAACCAAATGCACTTCGT
R: GGCTGCCGTAGAGGTATGGGA
Adapted from Ref. [17]
IFNG Interferon γ F: ACACTGACAAGTCAAAGCCGC
R: AGTCGTTCATCGGGAGCTTG
Adapted from Ref. [18]
IFNB Interferon β F: ACCAGATCCAGCATTACATCCA
R: CGCGTGCCTTGGTTTACG
Adapted from Ref. [19]
IL1B Interleukin 1 β F: GGAGGTTTTTGAGCCCGTC
R: TCGAAGATGTCGAAGGACTG
Adapted from Ref. [15]
IL2 Interleukin 2 F: GCTTATGGAGCATCTCTATCATCA
R: GGTGCACTCCTGGGTCTC
Adapted from Ref. [20]
IL4 Interleukin 4 F: GCTCTCAGTGCCGCTGATG
R: GGAAACCTCTCCCTGGATGTC
Adapted from Ref. [19]
IL6 Interleukin 6 F: AGGACGAGATGTGCAAGAAGTTC
R: TTGGGCAGGTTGAGGTTGTT
Adapted from Ref. [21]
IL8 (IL8L2) Interleukin 8 F: AAGGATGGAAGAGAGGTGTGCTT
R: GCTGAGCCTTGGCCATAAGT
Adapted from Ref. [19]
IL10 Interleukin 10 F: CATGCTGCTGGGCCTGAA
R: CGTCTCCTTGATCTGCTTGATG
Adapted from Ref. [22]
IL12 (IL12B) Interleukin 12 F: TTGCCGAAGAGCACCAGCCG
R: CGGTGTGCTCCAGGTCTTGGG
Adapted from Ref. [18]
IL17 Interleukin 17 F: CCGTCTTCTGCTGAGAGGAGTG
R: ACCGTTGTTCCGTCCCATCAC
Adapted from Ref. [20]
TNFAIP6 Tumor necrosis factor-inducible gene 6 protein F: CTGGCTGTCCCTGTGTGATT
R: TCAGGTGCTATTGCTGCGAG
Adapted from Ref. [5]
NCF1C Neutrophil Cytosolic Factor 1C F: CTGTGGATGGTGTCACCGAA
R: TGCCATTCTCACAGCCCTAC
Adapted from Ref. [5]
AvBD1 (GAL2) Avian β-defensin 1 F: AAACCATTGTCAGCCCTGTG
R: TTCCTAGAGCCTGGGAGGAT
Adapted from Ref. [15]
CATHL2 (CAMP) Cathelicidin F: AGGAGAATGGGGTCATCAGG
R: GGATCTTTCTCAGGAAGCGG
Adapted from Ref. [15]
MUC6 Mucin 6 F: TTCAACATTCAGTTCCGCCG
R: TTGATGACACCGACACTCCT
Adapted from Ref. [15]
CLDN1 Claudin 1 F: TCTTCATCATTGCAGGTCTGTC
R: AACGGGTGTGAAAGGGTCAT
Adapted from Ref. [15]
TJAP1 Tight junction-associated
protein 1
F: AGGAAGCGATGAATCCCTGTT
R: TCACTCAGATGCCAGATCCAA
Adapted from Ref. [15]