Table 1.

Primers and PCR conditions used for Plasmodium detection.

PCR Reaction	Primer Name	Primer Sequence (5′-3′)	Annealing Temperature	Amplicon Size (bp)
Genus Plasmodium first PCR	rPLU1	TCA AAG ATT AAG CCA TGC AAG TGA	55 °C
	rPLU5	CCT GTT GTT GCC TTA AAC TYC
Genus Plasmodium nested PCR	rPLU3	TTT YTA TAA GGA TAA CTA CGG AAA AGC TGT	62 °C	240
	rPLU4	TAC CCG TCA TAG CCA TGT TAG GCC AAT ACC		107
Plasmodium vivax	rVIV1	CGC TTC TAG CTT AAT CCA CAT AAC TGA TAC	58 °C
	rVIV2	ACT TCC AAG CCG AAG CAA AGA AAG TCC TTA		205
Plasmodium falciparum	rFAL1	TTA AAC TGG TTT GGG AAA ACC AAA TAT ATT	58 °C
	rFAL2	ACA CAA TGA ACT CAA TCA TGA CTA CCC GTC