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. 2022 Apr 26;13(5):765. doi: 10.3390/genes13050765

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Silencing GhPRA1.B1-1A weakened V. dahliae resistance in upland cotton plants. (A) The photobleached phenotype after infection by TRV:GhCLA1. (B) Detection of the expression levels of the GhPRA1.B1-1A gene in GhPRA1.B1-1A-silenced plants. (C) Phenotypes of control (TRV:00) and TRV:GhPRA1.B1-1A plants. (D) DI after 21 days of Vd991 infection. (E) Observation of the browning degree in stem vascular bundles. (F,G) The degree of fungal colonization and relative fungal biomasses of TRV:00 and TRV:GhPRA1.B1-1A. (H) At 48 h after inoculation, the levels of ROS were detected by DAB; bar = 200 μm. (I) Callose deposition in TRV:00 and TRV:GhPRA1.B1-1A cotton leaves; bar = 200 μm. (J) JA contents of TRV:00 and TRV:GhPRA1.B1-1A. (K) SA contents of TRV:00 and TRV:GhPRA1.B1-1A. (L) H₂O₂ contents of TRV:00 and TRV:GhPRA1.B1-1A. The difference was statistically significant (** p < 0.01).