Glucose and maltose accumulation in the wort during mashing experiments with barley malt and starch-rich T. chui biomass. (a,b) Mashing with barley malt; B1-4 correspond, respectively, to 2.88, 3.15, 3.42 and 3.6 g of biomass in 15 mL. (c,d) Mashing with starch-rich T. chui biomass; M1-3 correspond to, respectively, 0.72, 0.45 and 0.18 g of biomass in 15 mL. (e,f) Mashing with starch-rich T. chui biomass and barley malt mixtures without pH adjustment; 20% (MB1), 12.5% (MB2) and 5% (MB3) microalgal biomass was used over 3.6 g of total solids in 15 mL. (g,h) Mashing with starch-rich T. chui biomass and barley malt mixtures with pH adjustment; 20% (MB1), 12.5% (MB2) and 5% (MB3) microalgal biomass was used over 3.6 g of total solids in 15 mL. Samples were incubated over five hours at 67 °C. Detailed information regarding samples is described in Figure 1. All data represent mean ± SD of triplicate measurements.