. 2022 Apr 27;13(5):777. doi: 10.3390/genes13050777

Table 2.

Comparison of protein interactors of well-characterized HORMAD proteins MAD2 and REV7 with meiotic HORMADs. * Proteins indicated have been suggested to interact via the safety belt-CM mechanism, based on sequence similarities and evidence of a direct interaction. ^‡ Kd measurements have used truncated proteins, consisting of just their HORMA domain. ^† Proteins indicated have been characterized interacting outside of the HORMA domain. ^# No evidence for direct interaction. Immunoprecipitation and/or fluorescence colocalization dependency suggests an interaction. Background color means MAD2 and REV7 are not meiotic HORMADs.

.	Protein	Additional Structural Features	Interactors through Safety Belt-CM	Kd/μM	Interactors through an Alternate Interface	Interactors through an Uncharacterized Interface	References
H. sapiens	MAD2	-	MAD1	1.04	MAD2	-	[25,37,98,99]
			CDC20	0.1	p31^comet		[26,98,100]
			SGO2	0.69	TRIP13		[32,101]
			REV3 *		BUBR1		[102,103]
			RIT1 *		WT1		[104,105]
H. sapiens	REV7	-	SHLD3	0.013 ± 0.0004	REV7	CLTA	[28,36,106,107]
			RAN	1.85	MAD2	HCCA2	[36,108,109]
			REV3		p31^comet	PRCC	[35,36,110,111]
			IpaB		REV1	SIM2	[28,38,108,112]
			CAMP		SHLD2	TCF4	[28,39,113,114]
			ELK-1 *		TRIP13	ADP/E3-11.6K	[39,115,116]
			MDC9 *		CDH1	TF11-1	[102,117,118]
S. cerevisiae	Hop1	C-ter CM	Hop1 *	6.1 ± 1 ^‡	Pch2	Mer2	[27,60,71]
		zinc finger	Red1	0.34 ± 0.03 ^‡	Mek1 ^†	PP4 ^#	[27,92,119]
S. pombe	Hop1	C-ter CM	Rec10 *		Rec15 ^†		[43]
		zinc finger
H. sapiens	HORMAD1	C-ter CM	HORMAD1 *				[40]
			HORMAD2 *				[40]
			MCM9 *				[120]
M. musculus	HORMAD1	C-ter CM			TRIP13 ^#	Cohesin ^#	[10,33,45]
						IHO1	[70]
	HORMAD2	C-ter CM	HORMAD2 *	7.1 ± 0.5 ^‡			[44]
			SYCP2 *				[44]
			HORMAD1 *				[10]
C. elegans	HTP-1	C-ter CM	HIM-3	0.7		LAB-1 ^#	[19,40,88]
			HTP-3 motif #1	0.3			[40,88]
			HTP-3 motif #6 *	0.9			[40,88]
	HTP-2	C-ter CM	HIM-3	3.1			[40]
			HTP-3 motif #1	0.2			[40]
			HTP-3 motif #6	0.3			[40]
	HIM-3	C-ter CM	HTP-3 motif #4	0.3			[40,88]
			HTP-3 motifs #2, 3, 5 *				[40,88]
	HTP-3	6 C-ter CM				MRE-11/RAD-50 ^#	[14]
						Cohesin ^#	[40]
A. thaliana	ASY1	C-ter CM	ASY1 *		COMET		[44,57,58]
		SWIRM domain	ASY3 *		PCH2 ^#		[41,44]
	ASY2						[121]
Oryza sativa	PAIR2	C-ter CM SWIRM domain	PAIR3 *		CRC1 (PCH2)		[56,122]