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. 2022 May 23;12(5):1291. doi: 10.3390/diagnostics12051291

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Graphical representation of parameters in FFPE DNA samples according to NGS coverage. (a): DIN, (b): DNA concentration, (c): DNA library concentration (Left to right). Figure 3 shows a box plot of FFPE DNA sample coverage with respect to parameters such as DIN, DNA concentration, and library concentration. All the parameters show a significant difference between the poor coverage (below 250× and 10% VAF) and good coverage (above 250× and 10% VAF). * represents the extreme data points, ◦ represents the ouliers data points.