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. 2022 May 23;12(5):1291. doi: 10.3390/diagnostics12051291

Table 1.

Sample characteristics of FFPE DNA according to NGS coverage.

FFPE DNA
Parameters ≤250× and VAF 10% (Poor Coverage) (n = 46) ≥250× and VAF 10% (Good Coverage) (n = 98) p-Value
Minimum Median Maximum Minimum Median Maximum
DNA Integrity Number (DIN) 1.3 2.1 6 3 4.8 7 <0.05
Initial FFPE DNA concentration (ng/µL) 2.39 4.99 53 5.9 39.175 764 <0.05
Library concentration (nanomol) 0.37 3.955 27.65 40.3 137.18 325 <0.05