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. 2022 May 23;12(5):1291. doi: 10.3390/diagnostics12051291

Table 2.

Sample characteristics of FFPE RNA according to NGS coverage.

FFPE RNA
Parameters ≤250× and VAF 5% (Poor Coverage) (n = 64) ≥250× and VAF 5% (Good Coverage) (n = 39) p-Value
Minimum Median Maximum Minimum Median Maximum
RIN 1.1 2.3 5.6 1.2 2.3 4.3 0.948
RNA input for cDNA 12.67 299.95 3927 14.84 327.6 3437 0.21
DV100 9.15 66.4 86.77 18.25 71.055 91.04 0.128
DV200 2.24 42.91 76.44 10.61 49.13 79.46 0.095
RNA Library Concentration Nanomol 0.716 11.14 292 6.4 79.715 284.09 <0.05