Table 3.
Correlation of pre-analytical and post-analytical parameters with coverage status.
| Parameters | Correlation Coefficient w.r.t NGS Coverage |
p-Value |
|---|---|---|
| FFPE DNA | ||
| DNA Integrity Number (DIN) | 0.703 ** | <0.01 |
| FFPE DNA concentration (ng/µL) | 0.717 ** | <0.01 |
| Library concentration (nanomole) | 0.808 ** | <0.01 |
| FFPE RNA | ||
| RIN | 0.006 | 0.949 |
| RNA input for cDNA (ng) | 0.124 | 0.211 |
| DV100 | 0.151 | 0.128 |
| DV200 | 0.165 | 0.095 |
| RNA Lib Conc Nanomole | 0.520 ** | <0.01 |
** = Correlation is significant at the 0.01 level. All correlations were analyzed using Spearman’s rho test. The DNA preanalytical parameters are DIN and DNA concentration (ng/µL) while for RNA, RIN, RNA input for cDNA (ng), DV100, and DV200 are preanalytical steps. The post-analytical parameter for DNA and RNA is library concentration (nanomole).