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NSC34 differentiate in 14 days of culture. NSC34 were differentiated using DMEM High Glucose, 1% penicillin/streptomycin, 1% L-glutamine and 10 μM all-trans retinoic acid (RA), and the differentiation process was evaluated at days 0, 1, 4, 7, 11 and 14 using the optical microscope Axio Imager 2 (Zeiss, Oberkochen, Germany). Cells began to show propagation already at day 1 and then grouped into colonies with many interconnections. Scale bar = 25 µm.
