Table 2.
List of RT-qPCR primers used for the detection of the differentiation step markers.
| Gene | Primer Sequences (5′-3′) | Annealing Temperature |
|---|---|---|
| Nestin |
F GGA AGA GAA CCT GGG AAA GG R GAT TCA GCT CTG CCT CAT CC |
60 °C |
| SOX2 |
F AGTCTCCAAGCGACGAAAAA R TTTCACGTTTGCAACTGTCC |
60 °C |
| SOX1 |
F AAATACTGGAGACGAACGCC R AACCCAAGTCTGGTGTCAGC |
60 °C |
| PAX6 |
F TGTGTGCTCTGAAGGTCAGG R CTGGAGCTCTGTTTGGAAGG |
60 °C |
| TUBB3 |
F CAGATGTTCGATGCCAAGAA R GGGATCCACTCCACGAAGTA |
60 °C |
| MAP2 |
F AGGGCTGGTAGGTTGGATCT R TGTGTCTCTGCCTTTGTATC |
60 °C |
| GAPDH |
F CAG CAA GAG CAC AAG AGG AAG R CAA CTG TGA GGA GGG GAG ATT |
60 °C |