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SDS-PAGE of LigY hydrolase produced in E. coli. Proteins (10 μg each) were separated on an SDS–12% PAGE gel and stained with Coomassie brilliant blue. Lanes: 1, molecular mass standard proteins; 2, E. coli MV1190(pBluescript II KS+); 3, E. coli MV1190(pHE36).
