Figure 1.

RNAlater (Qiagen) injection into the bile duct (BD). (A) The liver was flipped over and pushed aside to expose the pancreas (P). (B) The BD was located and traced distally to its point of joining with the pancreatic duct (hepatopancreatic duct: HPD) at the sphincter of Oddi and occluded with a clamp at the clamp site (CS). RNAlater (Qiagen) was injected by inserting a diabetic syringe needle into the BD. (C) To confirm the effectiveness of the RNAlater (Qiagen) perfusion technique, Indian ink was injected into a test pancreas, showing good perfusion. The diagrams in the figure were generated using mindthegraph.com. Accessed on 10 December 2021.