Table 2.
Quantification of RNA from rat pancreas following different excision techniques and extraction protocols.
| RNA Extraction Method | Modification(s) | Sample Source | RIN | Conc. ng/µL | Absorbance Ratio A260/A280 | Absorbance Ratio A260/A230 |
|---|---|---|---|---|---|---|
| RNeasy Mini Kit | - | NR | 3.6 ± 0.8 | 812.7 ± 30.1 | 1.95 ± 0.08 | 1.49 ± 0.24 |
| TriPure | RNAlater pancreatic injection after excision | NR | 3.7 ± 0.8 | 1263.0 ± 387.4 | 1.78 ± 0.14 | 1.00 ± 0.13 |
| RNeasy Mini Kit | RNAlater injection into BD without clamping + homogenization with 5 mL QIAzol | NR | 7.3 ± 0.5 | 1640.7 ± 436.3 | 2.12 ± 0.01 | 2.01 ± 0.07 |
| RNeasy Mini Kit | RNAlater injection into BD with clamping + homogenization with 5 mL QIAzol + centrifugation after tissue homogenization | NR | 8.1 ± 0.1 | 391.0 ± 55.9 | 2.09 ± 0.01 | 1.96 ± 0.05 |
| RNeasy Mini Kit | RNAlater injection into BD with clamping + homogenization with 7 mL QIAzol + centrifugation after tissue homogenization | NR | 7.2 ± 0.2 | 595.7 ± 164.9 | 2.11 ± 0.01 | 1.91 ± 0.08 |
Results are reported as mean ± SEM. BD, bile duct. RIN, RNA integrity number; NR, normal healthy rats; TriPure isolation reagent (Roche, Basel, Switzerland).