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. 2022 May 13;23(10):5479. doi: 10.3390/ijms23105479

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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MTT assay in GSCs and GBM cells; (A) U87, (B) GBM1, (C) LN229, and (D) NCH644. The majority of groups have been affected considerably by Au-PEG-CD133 and Au-PEG-CD133-CB-839 compared to Au and Au-PEG. The results have shown the significant efficacy of Au-PEG-CD133-CB-839 on most cohorts (GBM1, U87and NCH-644) in concentrations of approximately 20 nM, which could show the appropriate efficiency of our nanomedicines in low concentration compared to naïve CB-839, Au-Cit and Au-PEG-COOH as our control groups of nanomedicines. The cell line LN229 exhibited high resistance to GLS1 inhibition interventions.