Figure 1.

Identification of the DUB that promotes the proliferation of esophageal cancer cells. (A) Each of the 98 human DUB genes was suppressed in KYSE30 cells with pooled siRNAs and the cell proliferation was detected by the CCK-8 assay. (B) Knockdown efficiency of PSMD14 and USP39 in KYSE30 cells was examined by qRT-PCR. (C) Tumor volumes of USP39 silenced KYSE30 cells and control cells. n = 7 mice per group. (D) Tumor volumes of PSMD14 silenced KYSE30 cells and control cells. n = 7 mice per group. (E) Representative images of IHC staining of USP39 in USP39 high expression or USP39 low expression cases. Scale bars, 200 $\mu$m. (F) Kaplan–Meier analysis of ESCC patients’ overall survival and disease-free survival grouped by low or high expression of USP39 by the log rank test. Data in A, B, C, D are shown as the mean ± S.D. Data were analyzed by one-way ANOVA with Bonferroni correction (A,B) or two-way ANOVA with Bonferroni correction (C,D). ** p < 0.01, *** p < 0.001, **** p < 0.0001.