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. 2022 May 3;13(5):819. doi: 10.3390/genes13050819

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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USP39 interacts with several spliceosome components. (A) Overexpression efficiency of USP39 in KYSE30 cells was determined by Western blot. (B) The USP39 stable expression clone was established. Proteins that interacted with USP39 were purified from KYSE30 cells expressing Flag-tagged USP39 or vector control. (C) GO enrichment analysis of proteins pulled down by Flag antibody from KYSE30 cells expressing Flag-tagged USP39. (D) KEGG pathway analysis of proteins pulled down by Flag antibody from KYSE30 cells expressing Flag-tagged USP39. (E) The interaction between USP39 and core spliceosome factors was validated by Co-IP in KYSE30 cells.