Figure 3.

Suppressed apoptosis rate was exhibited in radioresistant CaSki and C33A cells, and overexpression of microRNA-29a triggered the apoptosis in radioresistant cervical cancer cells. (A) Representative figure of flow cytometric analysis Annexin-V/PI double staining was executed on WT- or RR-CaSki cells, which were pre-transfected with Scramble NC, miR-29a AS, or Pre-miR29a, and then were subjected to the 6-Gy radiation treatment and the apoptosis rate was estimated, as described in Section 4.6. Summary of apoptotic cells (sum of Annexin-V-positive/PI-negative and Annexin-V-positive/PI-positive cells) over 48 h after 6-Gy radiation treatment. (B) CaSki cells or (C) C33A cells. Data were obtained from three independent experiments in triplicate and shown as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.