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. 2022 May 15;23(10):5524. doi: 10.3390/ijms23105524

Figure 4.

Figure 4

Radioresistant (RR) CaSki and C33A cells exhibited augmented migration in vitro, and overexpression of miR-29a effectively abrogated RR-augmented cell migration. (A) Representative phase-contrast micrographs depicting scratched monolayers of serum-starved WT and RR CaSki and C33A cells at 0 and 8 h. Scale bar = 100 μm. The results of each cell were repeated at least three times, and the results were similar. (B) Summary of wound healing 8 h after scratching. Data are shown as mean ± SD. (C) Representative cell migration trace from a time-lapse recording of cell migration over 8 h. The results are representative of C33A and CaSki cell clones, and each clone was repeated at least three times. Scale bar = 100 μm. (D) Summary of migration speed from a time-lapse recording of cell migration over 8 h. Data are the mean ± SD of three independent experiments. All radioresistant C33A and CaSki cell clones underwent stable selection for 6 months after irradiation, and >20 cells per individual sample were counted. * p < 0.05, ** p < 0.01, *** p < 0.001.