Table 1.
Primers used to test for rickettsiae in this study.
| Gene | Primers | Nucleotide Sequence (5′-3′) | Amplicon Size | Reference |
|---|---|---|---|---|
| Citrate synthase | RrCS.372 RrCS.989 |
TTTGTAGCTCTTCTCATCCTATGGC CCCAAGTTC CTTTAATACTTCTTTGC |
617 bp | [47] |
| Citrate synthase | RpCs.877p RpCs.1258n |
GGGGGCCTGCTCACGGCGG ATTGCAAAAAGTACAGTGAACA |
381 bp | [48] |
| rOmpB | 120-M59 120-807 |
CCGCAGGGTTGGTAACTGC CCTTTTAGATTACCGCCTAA |
862 bp | [49,50] |
| OmpA | Rr190-70 Rr190-701 |
ATGGCGAATATTTCTCCAAAA GTTCCGTTAATGGCAGCATCT | 632 bp | [51] |
| OmpA | Rr190-70 Rr190-701 Rr190-602 |
ATGGCGAATATTTCTCCAAAA GTTCCGTTAATGGCAGCATCT AGTGCAGCATTCGCTCCCCCT |
550 bp | [41] (modified for touchdown PCR in this study) |