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Effect of cell concentration on the electrotransformation of R. leguminosarum with plasmid pMP154a
| Cell concentration (CFU/ml) | Total transformants, 108 |
|---|---|
| 1.0 × 108 | 0.10 |
| 3.0 × 108 | 1.40 |
| 9.0 × 108 | 1.80 |
| 2.7 × 109 | 2.00 |
| 8.0 × 109 | 5.40 |
| 2.4 × 1010 | 9.60 |
Aliquots of the desired cell concentration were prepared and electroporated at a field strength of 14.0 kV/cm with a pulse length of 7.3 ± 0.4 ms.
