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. 2022 May 27;11:e74637. doi: 10.7554/eLife.74637

Figure 5. Glutamatergic lateral horn neurons (LHNs) receive their major excitatory input from uniglomerular projection neurons (uPNs) and an odorant-selective inhibition from multiglomerular projection neurons (mPNs).

Upper panel schematic represents the experimental approach: the iACT tract was laser transected while odor-evoked responses were monitored from glutamatergic LHNs. (A, A’, A’’) Representative images and graphical comparison of responses evoked by vinegar in glutamatergic LHNs before and after laser transection of the iACT, across three different planes (n = 6). (B, B’, B’’) Representative images and graphical comparison of responses evoked by benzaldehyde in glutamatergic LHNs before and after laser transection of the iACT (n = 6). Lower panel schematic represents the experimental approach: the mACT tract was laser transected while odor-evoked responses were measured from glutamatergic LHNs. (C, C’, C’’) Representative images and graphical comparison of vinegar-evoked responses of glutamatergic LHNs before and after laser transection of the mACT (n = 10). (D, D’, D’’) Representative images and graphical comparison of benzaldehyde-evoked responses of glutamatergic LHNs before and after laser transection of the mACT (n = 10) (paired t-test ***p<0.001, *p<0.05). The red and green circles indicate individual data points for benzaldehyde and vinegar, respectively. The black circles indicate the averaged response.

Figure 5—source data 1. Summary of analyzed data to plot Figure 5.

Figure 5.

Figure 5—figure supplement 1. Laser transaction does not affect activity of glutamatergic lateral horn neurons (LHNs) in the intact brain hemisphere.

Figure 5—figure supplement 1.

(A) Comparison of benzaldehyde-evoked activity in glutamatergic LHNs in the intact hemisphere, before and after laser ablation of the iACT in the other, treated brain hemisphere (n = 6) (paired t-test, p=0.19). (B) Comparison of benzaldehyde (n = 10) and 2,3 butanedione (n = 8)-evoked activities in glutamatergic LHNs in the intact hemisphere, before and after laser ablation of the mACT in the other hemisphere (paired t-test, p=0.084 for benzaldehyde and p=0.37 for 2,3 butanedione). The red and green circles indicate individual data points for benzaldehyde and 2,3 butanedione, respectively. The black circles indicate the averaged response.
Figure 5—figure supplement 1—source data 1. Summary of analyzed data to plot Figure 5—figure supplement 1.
Figure 5—figure supplement 2. Multiglomerular projection neuron (mPN)-mediated odor-selective inhibition onto glutamatergic lateral horn neurons (LHNs).

Figure 5—figure supplement 2.

Comparison of glutamatergic LHN responses in the middle plane before and after laser transection of the mACT reveals a significant increase in activity only in the case of acetophenone (p=0.00059), whereas a trend was observed for other odors (2,3 butanedione [p=0.1], ethyl acetate [p=0.13], and linalool [0.083]) (paired t-test, n = 10). The red and green circles indicate individual data points for aversive odors (acetophenone and linalool) and attractive odors (2,3 butanedione and ethyl acetate), respectively. The black circles indicate the averaged response.