Video 3. A representative primary B cell expressing GFP-F-Tractin on glass coated with anti-IgM and ICAM-1 that was imaged every 3 s for 300 s using TIRF-SIM (A).

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A magnified view of the region boxed in white in (A) is shown in (B).Played back at 10 fps. Scale bars: 5 μm (A), 1 μm (B).