Figure 9.

Protein expression of Na⁺ transporters in Dahl salt-sensitive rats after chronic dapagliflozin administration. A: Western blot analysis of Na⁺/H⁺ exchanger isoform 3 (NHE3), Na⁺-glucose cotransporter-2 (SGLT2), Na⁺-K⁺-2Cl⁻ cotransporter (NKCC2), phosphorylated (p)NKCC2, Na⁺-Cl⁻ cotransporter (NCC), pNCC, and α-, β-, and γ-subunits of the epithelial Na⁺ channel (ENaC). Each line represents 1 rat. B and C: summary graphs showing the average relative density of the bands (normalized to loading controls) in the studied groups. Quantification of protein expression was performed by normalizing to the corresponding loading control. Shown in Supplemental Fig. S2 are other blots for NKCC2 and additional loading controls. Results obtained from untreated rats were designated as 1. n ≥ 5. ns, not significant.