Table 1.

Primer sequences used for RT-PCR analysis

Target Gene	Protein	Strand	Sequence (5′−3′)	Product Size, bp
Slc5a2	SGLT2	Forward	GGTGTTGGCTTGTGGTCTATGT	134
		Reverse	ACAAAATGACCGCTGCCGAT
Slc12a1	NKCC2	Forward	AAAGGTGTGCTGGTGAGGTG	131
		Reverse	GAGGTTACCATGGTGGAAAGAAG
Slc12a3	NCC	Forward	GTGGCTGAACAAGAGGAAGA	212
		Reverse	AGTTGAAGTCAAAGGCATCG
Scnn1a	α-ENaC	Forward	CCCTGCAACCAGGCGAATTA	209
		Reverse	TCCTGACCATGCACCATCAC
Scnn1b	β-ENaC	Forward	CAGCTTTCTAAACAGGTGCCA	114
		Reverse	TGCAGTACCACACTAGCAGC
Scnn1g	γ-ENaC	Forward	TCACGCTTTTCCACCATCCA	113
		Reverse	GATGACTTGCAGCCCGTACT

SGLT2, Na⁺-glucose cotransporter-2; NKCC2, Na⁺-K⁺-2Cl⁻ cotransporter; NCC, Na⁺-Cl⁻ cotransporter; ENaC, epithelial Na⁺ channel.