Figure 4.

Gene signatures from EMT-induced ALI cultures overlap those from different lung diseases. A: expression or immune associated genes in RNA-Seq data from ALI cultures treated with or without EMT cocktail, identified by Gene Ontology (GO) analysis. n = 8 donors. B: Ingenuity Pathway Analysis (IPA) of genes differentially expressed between untreated (control) and EMT-treated ALI cultures, compared with custom built disease gene lists. Plotted are P values for enrichment scores. C: Venn diagram for the genes identified from IPA analysis in asthma, IPF, and COPD. Listed are differentially expressed genes between untreated (control) and EMT-treated ALI cultures that also overlap all three diseases. ALI, air-liquid interface; COPD, chronic obstructive pulmonary disease; EMT, epithelial-mesenchymal transition.