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. 2022 May 20;2022:6852661. doi: 10.1155/2022/6852661

Figure 5.

Figure 5

Exosome-delivered miR-223-3p mediated the protective effect of EVs in H/R-stimulated TCMK-1 cells. TCMK-1 cells were subjected to H/R stimulation and miR-223-3p-depleted exosome treatment. (a) The H/R-induced cell apoptosis was determined using flow cytometry. (b) The protein levels of BAX, cleaved caspase-3, and Bcl-2 were confirmed by performing the western blot analysis. (c) Expressions of the mitophagy markers were detected using the western blot analysis. (d) Mitophagosome formation was assessed based on the colocalization of the autophagy marker GFP-LC3B and the mitochondria marker Mito Tracker (DsRed-Mito). (e) The levels of mitochondrial DNA (mtDNA) were determined through qPCR. (f) The expressions of NLRP3, ASC1, and cleaved caspase 1 were evaluated using the western blot analysis. N = 3. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.