Fig. 2.

Overexpression of miR-26b-5p alleviated OGD/R-induced injury. a miR-26b-5p expression levels in Mode-k cells following the transfection of miR-26b-5p mimic or negative control (mimic-NC) constructs (n = 3). b Mode-K cell viability was measured via CCK8 assay. Mode-K cells were transfected with miR-26b-5p mimic or mimic-NC constructs before OGD treatment for 4 h and a 4 h reperfusion period (n = 5). c Representative TUNEL staining and apoptotic index values for Mode-k cells that underwent OGD/R (n = 5). Scale bar = 100 μm. d The rate of Mode-K cell apoptosis was measured by flow cytometry for cells that underwent OGD/R after transfection (n = 5). e The protein levels of C-caspase3 (cleaved-caspase3), Bcl2, and Bax in Mode-k cells that underwent OGD/R after transfection (n = 3). All data are expressed as the mean ± SD. *P < 0.05, **P < 0.01 versus control group