Table 1.
Phenotypical parameters of WT, Clcn2R180Q/+ and Clcn2−/− mice.
| Parameter | WT | R180Q | KO | Female WT | Female R180Q | Male WT | Male R180Q |
|---|---|---|---|---|---|---|---|
| Plasma aldosterone (pg ml−1) | 242.5 ± 17.57 (N = 37) | 356.3 ± 23.96**** (N = 36) | 254.5 ± 22.36 (N = 10) | 267.4 ± 22.63 (N = 21) | 361.5 ± 28.98* (N = 23) | 209.8 ± 26.29 (N = 16) | 347 ± 43.61** (N = 13) |
| PRC (ng ml−1 h−1) | 1474 ± 245.3 (N = 37) | 1334 ± 138.7 (N = 36) | 2602 ± 546.1* (N = 10) | 1839 ± 392 (N = 21) | 1589 ± 166.3 (N = 23) | 995.1 ± 194.7 (N = 16) | 882.6 ± 198 (N = 13) |
| ARR (pg ml−1: ng ml−1 h−1) | 0.25 ± 0.03 (N = 37) | 0.4 ± 0.05 (N = 36) | 0.12 ± 0.02 (N = 10) | 0.23 ± 0.03 (N = 21) | 0.29 ± 0.04 (N = 23) | 0.26 ± 0.04 (N = 16) | 0.59 ± 0.12*** (N = 13) |
| Plasma K+ (mmol l−1) | 5.01 ± 0.12 (N = 10) | 5.12 ± 0.13 (N = 16) | N/A | 4.78 ± 0.12 (N = 6) | 5.07 ± 0.19 (N = 10) | 5.34 ± 0.12 (N = 4) | 5.17 ± 0.15 (N = 6) |
| Plasma Na+ (mmol l−1) | 151.7 ± 0.57 (N = 10) | 152 ± 0.54 (N = 16) | N/A | 151.1 ± 0.78 (N = 6) | 150.9 ± 0.46 (N = 10) | 152.6 ± 0.7 (N = 4) | 153.7 ± 0.85 (N = 6) |
| Plasma Cl−(mmol l−1) | 111.9 ± 0.59 (N = 10) | 112.8 ± 0.52 (N = 16) | N/A | 112.9 ± 0.65 (N = 6) | 113.3 ± 0.52 (N = 10) | 110.4 ± 0.63 (N = 4) | 112.1 ± 1.07 (N = 6) |
| Urine K+ (mmol l−1) | 176.5 ± 10.3 (N = 13) | 166.5 ± 6.79 (N = 20) | N/A | 176.4 ± 17.02 (N = 7) | 156.8 ± 10.99 (N = 10) | 176.7 ± 12.08 (N = 6) | 176.1 ± 7.29 (N = 10) |
| Urine Na+ (mmol l−1) | 129.5 ± 15.31 (N = 13) | 82.02 ± 6.58** (N = 20) | N/A | 154.8 ± 19.55 (N = 7) | 88.69 ± 11.59** (N = 10) | 112.8 ± 12.42 (N = 6) | 67.71 ± 6.97 (N = 10) |
| Urine Cl− (mmol l−1) | 211.3 ± 12.65 (N = 13) | 161.9 ± 10.97* (N = 20) | N/A | 223 ± 15.55 (N = 7) | 147.9 ± 17.92** (N = 10) | 197.7 ± 20.61 (N = 6) | 175.9 ± 11.98 (N = 10) |
| SBP (mmHg) | N/A | N/A | N/A | N/A | N/A | 117.3 ± 0.1 (N = 11) | 120.2 ± 0.1* (N = 11) |
| DBP (mmHg) | N/A | N/A | N/A | N/A | N/A | 85.1 ± 0.1 (N = 11) | 87.3 ± 0.1* (N = 11) |
| HR (BPM) | N/A | N/A | N/A | N/A | N/A | 497.6 ± 0.7 (N = 11) | 516.1 ± 0.7* (N = 11) |
| MAP (mmHg) | N/A | N/A | N/A | N/A | N/A | 100.8 ± 0.1 (N = 11) | 103.3 ± 0.1** (N = 11) |
PRC plasma renin concentration, ARR aldosterone to renin ratio, SBP systolic blood pressure, DBP diastolic blood pressure, HR heart rate, BPM beats per minute, MAP mean arterial pressure
All values as mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.