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H3 cells and G4 ectopic sites are unstable under nonstressed growth conditions.A, map of short and long PCR templates. B, short PCR confirmation of H3 and G4 inserts. C, sequences of input and outcome H3 and G4 cassettes. H, non-G nucleotide. D, PCR analysis of H3, G4 recombination. Arrows, expected PCR product size (9.4 kb). G4, G-quadruplex; H3, triplex; NTC, no template control.
