Table 1.

Agent phenotypes, rules, and cell–cell interactions

Phenotype	Cell rules	Cell–cell interactions
Uninfected (glioblastoma)	Migration: cells were designated either “stop” or “go” with a corresponding time in each respective phenotype. Cells designated as “go” moved with Brownian motion (MODELLING CELL MORPHOLOGY AND KINETICS, STAR Methods) Proliferation: If not too crowded, cells will proliferate with some probability (CELL PROLIFERATION MODEL, STAR Methods). Infection: Cells uptake new virions from the extracellular milieu (VIRUS MODEL, STAR Methods).	Glioblastoma cell apoptosis: CD8+T cells induce apoptosis in infected glioblastoma cells if we assume sensitivity to tumor-antigen (IMMUNE CELL MODEL, STAR Methods).
Infected (glioblastoma)	Migration: cells were designated either “stop” or “go” cell with a corresponding time in each respective phenotype. Cells designated as “go” moved with Brownian motion (MODELLING CELL MORPHOLOGY AND KINETICS, STAR Methods). Death through lysis: cells undergo lysis if the newly replicated virions exceed $\alpha$ (VIRUS MODEL, STAR Methods).	Infected-cell apoptosis: CD8+ T cells induce apoptosis in infected glioblastoma cells (IMMUNE CELL MODEL, STAR Methods).
Dead (glioblastoma)	Disintegration: cell volume reduces mimicking fluid loss as pre-defined in PhysiCell rules (Ghaffarizadeh et al., 2018) (MODELLING CELL MORPHOLOGY AND KINETICS, STAR Methods).
Stromal	Virus uptake: Cells uptake virions from the extracellular milieu, but the virus does not replicate, and the cells do not lyse (VIRUS MODEL, STAR Methods).
Inactive CD4+ T cell	Migration: cells chemotaxis up chemokine gradient (MODELLING CELL MORPHOLOGY AND KINETICS, STAR Methods).	Activate: become activated through contact with infected glioblastoma cells (IMMUNE CELL MODEL, STAR Methods)
Active CD4+ T cell	Proliferation: Cells will proliferate with some probability (IMMUNE CELL MODEL, STAR Methods). Chemokine secretion: Secrete chemokines (IMMUNE CELL MODEL, STAR Methods)
Active CD8+ T cell	Migration: cells chemotaxis up chemokine gradient (MODELLING CELL MORPHOLOGY AND KINETICS, STAR Methods). Proliferation: Cells will proliferate with some probability (CELL PROLIFERATION MODEL, STAR Methods). Chemokine secretion: Secrete chemokines (IMMUNE CELL MODEL, STAR Methods).	Infected-cell apoptosis: induce apoptosis in infected glioblastoma cells (IMMUNE CELL MODEL, STAR Methods). Glioblastoma -cell apoptosis: induce apoptosis in infected glioblastoma cells if we assume sensitivity to tumor-antigen (IMMUNE CELL MODEL, STAR Methods).

For each agent type (glioblastoma, stromal, CD4⁺ and CD8⁺ T cells), the cell rules and cell–cell interactions are listed. For a full summary of the model rules, see Figure 2B and the STAR Methods.