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. 2022 May 27;13:2987. doi: 10.1038/s41467-022-30673-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Cryo-EM reconstruction of apo DrmAB (a) and DNA-bound DrmAB (b). Density corresponding to DrmA trigger loop is shown in pink. DrmAB are colored by structural domains as shown in Fig. 1a. c Overlay of DrmAB-bound DNA (blue) and TL (pink) showing the steric clash (dashed black & white outline). TL partially occludes DNA-binding site. DrmB and parts of DrmA have been omitted for clarity. Graphic at the top right shows how the view in (c) is related to the structures in panels (a, b). d, e EMSA analysis of DrmAB and DrmA(∆loop)B binding DNA. The smeared bands that occur at high concentrations of DrmAB correspond to bound DNA dissociating from the complex. This did not occur for DrmA(∆loop)B, and an additional super-shift was present, corresponding to multiple copies of DrmA(∆loop)B binding to the same 75-nt DNA concurrently. The free DNA marker is 75-nt in length. Representative of three independent experiments. Source Data are provided as a Source Data file. f, g DNA substrate preferences of DrmAB and DrmA(∆loop)B. The 75-nt Cy5-labeled DNA (ssDNA) was annealed to complementary oligos corresponding to the full sequence (dsDNA), or 20 bases at the 5’ or 3’ ends (3’ovh and 5’ovh, respectively). DrmAB showed a preference for ssDNA and 5’ovh DNA, whereas DrmA(∆TL)B bound to all DNA substrates tested. The free DNA marker is 75-nt in length. Representative of three independent experiments. Source Data are provided as a Source Data file. h Effect of truncation of TL on DISARM anti-phage activity. Points correspond to three biological replicates, with mean and standard deviation shown. Source Data are provided as a Source Data file.