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Southern blot analysis of Δnth1 ath1 double mutant. Total yeast genomic DNA was digested with EcoRI, electrophoresed on 1% agarose gels, transferred to a nylon membrane, and hybridized with a 1.4-kb EcoRI-KpnI fragment containing the NTH1 ORF (A) and a 1.1-kb fragment containing the ATH1 ORF prepared by PCR in which primer 1 and primer 2 (B) were used as probes. Lane 1, T118; lane 2, AT418; lane 3, T128; lane 4, AT428.
