Intracellular trehalose accumulation by trehalase mutants of T118 (A) and trehalase mutants of T128 (B). Samples were obtained at various growth phases, including the seed culture (SC), the early logarithmic phase (EL), the late logarithmic phase (LL), the early stationary phase (ES), and the late stationary phase (LS). (A) Symbols: ○, T118; ●, T154; ▵, A318; ▴, AT418. (B) Symbols: ○, T128; ●, T164; ▵, A328; ▴, AT428. The results are means ± standard deviations based on three independent experiments.