TABLE 2.
Neutral and acid trehalase activities of trehalase mutants
| Strain | Neutral trehalase activity (mU/mg of protein)a | Acid trehalase activity (mU/mg of protein)b |
|---|---|---|
| T118 | 96.0 | 13.5 |
| T154 | 18.0 | 13.5 |
| A318 | 62.0 | 1.0 |
| AT418 | 19.0 | 3.2 |
| T128 | 16.5 | 10.5 |
| T164 | 10.5 | 10.0 |
| A328 | 13.0 | 1.0 |
| AT428 | 10.0 | 1.0 |
a
Cells were grown in molasses medium and were harvested at the mid-logarithmic phase. One unit of neutral trehalase activity was defined as the amount of enzyme that catalyzed the hydrolysis of 1 μmol of trehalose/min at 30°C at pH 7.0.
b
Cells were grown in molasses medium and were harvested at the late stationary phase. One unit of acid trehalase activity was defined as the amount of enzyme that catalyzed the hydrolysis of 1 μmol of trehalose/min at 30°C at pH 4.5.