Figure 2.
Progesterone receptor stimulation drives proliferation and accumulation of maternal FOXP3+ cells
(A) FACS plots and summary graphs showing ratio of adoptively transferred PRKO [CD45.2+, CD45.1+] (red) to PRWT [CD45.2+, CD45.1−] (gray) FOXP3+ and FOXP3− maternal CD4+ splenocytes during allogeneic pregnancy (E11.5) sired by BALB/c males compared with virgin control mice.
(B) Expression of Ki-67, CD178 (Fas ligand), Bcl-2, Helios, Neuropilin, and percent dead cells identified by staining with a membrane integrity dye by donor PR-sufficient (FOXP3-WT) compared with PR-deficient (FOXP3-PRKO) FOXP3+ cells for mice described in panel A. Data are from at least three independent experiments, each with similar results, with each point representing data from an individual mouse. Bar, mean ± SEM. ∗p < 0.05, ∗∗∗p < 0.005, ∗∗∗∗p < 0.001.