REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
PE/Cy5 anti-mouse/human CD11b Antibody, Clone M1/70	BioLegend	Cat# 101210; RRID:AB_312793
PE/Cy5 anti-mouse/human CD45R/B220 Antibody, Clone RA3-B62	BioLegend	Cat# 103210; RRID:AB_312995
PE/Cy7 anti-mouse CD62L Antibody, Clone MEL-14	BioLegend	Cat# 104418; RRID:AB_313103
APC anti-mouse CD152 (CTLA-4) Antibody, Clone UC10-4B9	BioLegend	Cat# 106310; RRID:AB_2087653
Alexa Fluor 700 anti-mouse/human CD44 Antibody, Clone IM7	BioLegend	Cat# 103026; RRID:AB_493713
PE/Cy7 anti-mouse CD45.1 Antibody, Clone A20	BioLegend	Cat# 110730; RRID:AB_1134168
Brilliant Violet 421 anti-mouse CD45.2 Antibody, Clone 104	BioLegend	Cat# 109832; RRID:AB_2565511
eFluor 450 anti-mouse CD4 Antibody, Clone GK1.5	Invitrogen	Cat# 48-0041-82; RRID:AB_10718983
APC-eFluor 780 anti-mouse CD8alpha Antibody, Clone 53-6.7	Invitrogen	Cat# 47-0081-82; RRID:AB_1272185
PE/Cy5 anti-mouse CD11c Antibody, Clone N418	Invitrogen	Cat# 15-0114-82; RRID:AB_468717
PE/Cy5 anti-mouse F4/80 Antibody, Clone BM8	Invitrogen	Cat# 15-4801-82; RRID:AB_468798
FITC anti-mouse CD4 Antibody, Clone GK1.5	Invitrogen	Cat# 11-0041-82; RRID:AB_464892
PE/Cy7 anti-mouse CD8alpha Antibody, Clone 53-6.7	Invitrogen	Cat# 25-0081-82; RRID:AB_469584
PE/Cy7 anti-mouse CD3epsilon Antibody, Clone 145-2C11	Invitrogen	Cat# 25-0031-82; RRID:AB_469572
FITC anti-mouse FOXP3 Clone FJK-16S	Invitrogen	Cat# 11-5773-82; RRID:AB_465243
eFluor 450 anti-mouse Ki-67 Clone SolA15	Invitrogen	Cat# 48-5698-82; RRID:AB_11149124
APC anti-mouse/human Helios Clone 22F6	BioLegend	Cat# 137222; RRID:AB_10662900
eFluor anti-mouse Neuropilin-1 Clone 3DS304M	Invitrogen	Cat# 48-3041-82; RRID:AB_2574051
PE anti-mouse CD178 (Fas Ligand) Clone MFL3	BioLegend	Cat# 106606; RRID:AB_313279
AF647 anti-mouse Bcl-2 Clone 10C4	BioLegend	Cat# 633510; RRID:AB_2274702
APC anti-CD183 (CXCR3)	Invitrogen	Cat# 17-1831-82; RRID:AB_1210791
InVivoPlus anti-mouse CXCR3 Clone CXCR3-173	BioXCell	Cat# BE0249; RRID:AB_2687730
Chemicals, peptides, and recombinant proteins
H-2Kb:OVA257-64 MHC Class I Monomer	NIH Tetramer Core	N/A
Streptavidin, R-Phycoerythrin Conjugate (SAPE)	Invitrogen	S866
I-Ab:2W1S55-64 MHC Class II Tetramer	NIH Tetramer Core	N/A
Mifepristone, RU486	Sigma Aldrich	M8046
17alpha-hydroxyprogesterone caproate	Sigma Aldrich	H5752
LIVE/DEAD Near-IR staining kit	ThermoFisher	L34976
Critical commercial assays
Fixation/Permeabilization Solution Kit	BD Biosciences	554714
Anti-PE Microbeads	Miltenyi	130-048-801
Experimental models: Organisms/strains
Balb/c-Act-2W1S/OVA	Marc Jenkins (University of Minnesota)	Published in Moon et al. (2011)
Backcrossed 10 generations to BALB/c background
C57BL/6NCr	Charles River Laboratories (NCI)	#556
Balb/cAnNCr	Charles River Laboratories (NCI)	#555
B6-Ly5.1/Cr	Charles River Laboratories (NCI)	#564
B6.129(Cg)-Foxp3tm4(YFP/icre)/Ayr/J	Jackson Laboratory	#016959
PRflox/flox	Laboratory of John Lydon and Francisco De-Mayo	Published in Lydon et al. (1995)
Software and algorithms
FlowJo	BD Biosciences	N/A
GraphPad Prism	GraphPad Software	N/A