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. 2022 Apr 27;2(5):100205. doi: 10.1016/j.crmeth.2022.100205

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© 2022 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Non-uniform slow image distortions during in vivo two-photon calcium imaging

(A) Max-intensity projection image of an example RSC-imaging session. The max-intensity projection of all frames after correcting only rigid motion artifacts (left) and the max-intensity projections of the first (red) and the last (cyan) 14-min frames in the 3.5-h session after correcting only rigid motion artifacts (right) are shown. The second and third rows show the zoomed images that highlight the subfields where rigid corrections sufficiently (second row) or insufficiently (third row) registered early and late frames. The colors on the right panels are intentionally saturated with an arbitrary threshold to highlight the image overlaps.

(B) Max-intensity projection image of an example cholinergic axon-imaging session. The max-intensity projection of all frames after correcting only rigid motion artifacts (left) and the max-intensity projections of the first (red) and the last (cyan) 1.4-min frames in the 20-min session after correcting only rigid motion artifacts (right) are shown.