Fig. 6.

The effect of MitoQ supplementation on exercise-induced increases in skeletal muscle VEGF mRNA expression. A) Baseline skeletal muscle VEGF mRNA expression following 10 days of MitoQ or placebo supplementation. B) Representative blot of baseline skeletal muscle VEGF protein expression following 10 days of MitoQ or placebo supplementation and quantification of VEGF protein expression relative to GAPDH protein expression. Representative bands were run on the same gel and images have been cropped to show VEGF protein expression in participants from the placebo and MitoQ group together. C) Skeletal muscle VEGF gene expression immediately (Post) and 3 h (3 h) after exercise following 10 days of MitoQ or placebo supplementation presented as fold change from pre-exercise levels. Triangles and circles represent individual values. Differences in skeletal muscle VEGF mRNA and protein expression at baseline were analysed using unpaired Student's t-test. Main two-way repeated measures ANOVA effects are shown in figure C. *p < 0.05 for Fisher's LSD post hoc vs. Pre of the same group and #p < 0.05 for Fisher's LSD post hoc vs. placebo group at same time point. Data are presented as mean ± SD. VEGF; Vascular Endothelial Growth Factor, GAPDH; Glyceraldehyde 3-phosphate dehydrogenase.