TABLE 1.

Production of fumonisin B₁ by F. moniliforme cultured on maize kernels collected at four stages of reproductive development

Maize hybrida	Year	Reproductive stage	No. of days after silkingb	Moisture content (%)c	Fumonisin B1 production (mean ± SD)d
					μg/g (wet wt)e	μg/g (dry wt)f
Pioneer 3573	1995	Blister	8	84	18 ± 4 d	11 ± 3 c
		Milk	16	82	110 ± 25 c	61 ± 14 b
		Dough	26	55	420 ± 270 bg	93 ± 59 bg
		Dent	36	41	5,300 ± 1,500 a	900 ± 250 a
	1996	Blister	14	85	3 ± 3 d	2 ± 2 d
		Milk	23	72	200 ± 88 ch	68 ± 31 ch
		Dough	29	61	1,100 ± 590 b	240 ± 130 b
		Dent	35	44	5,900 ± 340 a	1,000 ± 58 a
Pioneer 3183	1995	Blister	13	77	36 ± 12 d	16 ± 5 c
		Milk	19	81	290 ± 80 c	150 ± 42 b
		Dough	26	59	740 ± 150 b	180 ± 37 b
		Dent	42	41	5,100 ± 1,200 a	870 ± 200 a
	1996	Blister	15	84	14 ± 18 d	9 ± 11 d
		Milk	22	77	41 ± 26 ch	18 ± 11 ch
		Dough	27	53	1,100 ± 400 b	230 ± 87 b
		Dent	35	42	6,100 ± 490 a	1,100 ± 400 a

^aPioneer Hybrids 3573 and 3183 are susceptible and tolerant, respectively, to Fusarium ear rot infection. 

^bNumber of days after silk emergence that ears were collected. 

^cThe moisture content of freshly harvested kernels was determined by drying a subsample of kernels in an oven overnight. 

^dThe values are means based on eight replicates per stage, for freshly harvested maize kernels (equivalent to 10 g [dry weight]) inoculated with F. moniliforme and incubated for 15 days. The fumonisin B₁ limit of detection was 1 μg/g. Means followed by a different letter within a column for a given hybrid and year are significantly different (P ≤ 0.05), as determined by the Student-Newman-Keuls test. Data were transformed to 1n (x + 1) values prior to analysis. 

^eFumonisin B₁ concentration calculated based on 10 g (wet weight) of maize kernel substrate. 

^fFumonisin B₁ concentration calculated based on 10 g (dry weight equivalent). 

^gThe dough stage means represent only four replicates due to contamination. 

^hDue to contamination in the original flasks, ears from an adjacent field plot that was planted 33 days later were collected and inoculated.