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Schematic representation of LevS and truncated forms. LevS: complete mature enzyme; LevSΔC: deletion of the C-terminal domain; LevSΔTnC: deletion of the transition region and the C-terminal domain; LevSΔN: deletion of the N-terminal domain; LevSΔNC: deletion of both N- and C-terminal domains; LevS-Cat: presence only of the catalytic domain. All proteins contain a histidine tag at the C-terminus.
