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. 1999 Jul;65(7):2857–2862. doi: 10.1128/aem.65.7.2857-2862.1999

TABLE 1.

Effect of growth mediuma on the membrane fatty acidb composition of Pediococcus cells grown at 28°C until ST or ME

Acyl chainc % Total fatty acid on:
TGY
TSB
TGYG
ST ME ST ME ST
SFA
 C13:0 0.09 0.12 ND 0.14 0.08
 C14:0 5.75 5.41 8.59 4.41 2.54
 C15:0 0.62 0.99 0.12 0.36 1.03
 C16:0 12.93 16.30 16.15 17.56 11.81
 C17:0 NDd ND ND 0.11 0.30
 C18:0 1.06 1.62 1.11 2.34 2.33
 C20:0 0.31 0.29 0.45 0.51 1.04
 Totale 20.76 ± 3.64 24.73 ± 2.28 26.41 ± 1.47 25.42 ± 4.44 19.13 ± 4.38
USFA
 C16:1 n9c 18.71 16.20 16.95 11.47 11.31
 C18:2 n9c, n12c 0.21 ND 0.92 0.19 ND
 C18:1 n9c ND 0.25 ND 0.39 ND
 C18:1 n11c 46.24 55.51 27.70 59.72 57.78
 Totale 65.16 ± 0.24 71.97 ± 1.21 45.56 ± 3.00 71.76 ± 3.74 69.09 ± 14.10
CFA
 C17:0 Δ9c 0.40 0.07 2.25 0.09 0.11
 C19:0 Δ9c 13.69 3.24 24.69 2.73 11.15
 Totale 14.10 ± 3.42 3.31 ± 1.09 26.93 ± 2.73 2.83 ± 0.74 11.26 ± 8.93
Others ND ND 1.09 ND 0.57
a

Cells were grown in 100 ml of TGY, TSB, or TGYG medium at 28°C until ST (A600 = 1.0) or ME (A600 = 0.5), centrifuged, and washed once with sterile distilled water. Wet cells (40 to 80 mg) were used in the preparation and analysis of FAME as described in Materials and Methods. 

b

Data are presented as percent total fatty acid and represent the mean of three replications. 

c

n, location of double bond; Δ, location of cyclopropane ring; c, cis

d

ND, not detected (species absent or below detection limit). 

e

Data are the total of the mean values ± the standard deviation of replicate values.