TABLE 1.

Effect of growth medium^a on the membrane fatty acid^b composition of Pediococcus cells grown at 28°C until ST or ME

Acyl chainc	% Total fatty acid on:
	TGY		TSB		TGYG
	ST	ME	ST	ME	ST
SFA
C13:0	0.09	0.12	ND	0.14	0.08
C14:0	5.75	5.41	8.59	4.41	2.54
C15:0	0.62	0.99	0.12	0.36	1.03
C16:0	12.93	16.30	16.15	17.56	11.81
C17:0	NDd	ND	ND	0.11	0.30
C18:0	1.06	1.62	1.11	2.34	2.33
C20:0	0.31	0.29	0.45	0.51	1.04
Totale	20.76 ± 3.64	24.73 ± 2.28	26.41 ± 1.47	25.42 ± 4.44	19.13 ± 4.38
USFA
C16:1 n9c	18.71	16.20	16.95	11.47	11.31
C18:2 n9c, n12c	0.21	ND	0.92	0.19	ND
C18:1 n9c	ND	0.25	ND	0.39	ND
C18:1 n11c	46.24	55.51	27.70	59.72	57.78
Totale	65.16 ± 0.24	71.97 ± 1.21	45.56 ± 3.00	71.76 ± 3.74	69.09 ± 14.10
CFA
C17:0 Δ9c	0.40	0.07	2.25	0.09	0.11
C19:0 Δ9c	13.69	3.24	24.69	2.73	11.15
Totale	14.10 ± 3.42	3.31 ± 1.09	26.93 ± 2.73	2.83 ± 0.74	11.26 ± 8.93
Others	ND	ND	1.09	ND	0.57

^aCells were grown in 100 ml of TGY, TSB, or TGYG medium at 28°C until ST (A₆₀₀ = 1.0) or ME (A₆₀₀ = 0.5), centrifuged, and washed once with sterile distilled water. Wet cells (40 to 80 mg) were used in the preparation and analysis of FAME as described in Materials and Methods. 

^bData are presented as percent total fatty acid and represent the mean of three replications. 

^cn, location of double bond; Δ, location of cyclopropane ring; c, cis. 

^dND, not detected (species absent or below detection limit). 

^eData are the total of the mean values ± the standard deviation of replicate values.